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文献和实验Immunohistochemisty-Fluorescence Protocol
/v) Saponin. Fluorescence Anti Fading Mounting Medium anti-fade mounting medium i-BRITE Plus (SF40000, Neuromics, Inc.) Saponin stock Prepare a stock of 10 (w/v) Saponin in EBSS. Prepare fresh, since crude saponin powder batches
Immunohistochemistry: Fluorescence Protocol 2.0
. Fluorescence Anti Fading Mounting Medium Carbonate/bicarbonate buffered glycerol (1:1 v/v) containing 2 % 1,4-Diazobicyclo 2.2 octane (Sigma Catalog # D2522) and adjust pH to 7.4. Saponin stock Prepare a stock of 10 % (w/v) Saponin in EBSS
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
) Dissect embryos or small pieces of tissue (e.g. the size of a retina from an adult rat works well with this procedure) into PBS containing 2mM MgCl2 (PBS + Mg2+) on ice. PBS(10X) 80 g NaCl 2 g KCl 11.5g Na2HPO4 2 g KH2PO
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