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文献和实验Mesoplasma florum: Tn5 Transposase
to Epicentre enzyme is OK) pWH1891 Sequence information T7 and Intein-R primers, ATG start at 45 Truncates aa''s 1-4 from canonical sequence Mutations E54K -- improve binding to OE M56A -- eliminate start for C
K Thomas R. Bürglin http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Search&db=PubMed&term=Burglin%20TR Christopher W.J. Smith http://embo-reports.oupjournals.org/cgi/content/full/2/12/1107 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Search&db
Screening lambda cDNA or genomic libraries
containing 500 µl 1X phage buffer.Vortex to release the phage. 2.In a 50 ml conical tube mix a)100 µl 2X cells b)200 µl of your phage stock c)10 µl of the M13 helper phage R408 (106-1011 pfu/ml) Incubate mixture at 37° for 15 minutes 3.Add 5 ml LB
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