- ￥50631
- Beckman Coulter贝克曼库尔特
- 美国
- 964382
- 2025年07月13日
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文献和实验Native Chromatin Preparation and Illumina/Solexa Library Construction
) Phusion HF Master Mix (2X, Illumina) (see Step 45) Polymerase chain reaction (PCR) primers PE 1.0 and PE 2.0 (Illumina) (see Step 45) Primer mix for real-time PCR (see Step 38) Several companies offer primer synthesis service.
AFLP: not only for fingerprinting, but for positional cloning
+ (no BSA) 1.0 µL (5-10 U/µL) T4 polynucleotide kinase (PNK) 24.0 µL dH2O §50 µL labeling rxn makes enough "hot" primer for 100 PCR rxns. † The basic EcoRI-primer is: 5'-AGA CTG CGT ACC AAT TCx yz-3' where, x, y, and z represent
AFLP: not only for fingerprinting, but for positional cloning
-primer is: 5'-AGA CTG CGT ACC AAT TCx yz-3' where, x, y, and z represent the selective bases on the 3' end of the oligo. [We typically use only 2 bp selective extensions (x and y, no z in our primers). x, y, and z are specific
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