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文献和实验protocol. Briefl y, disassociate cells from theplate, harvest by spin down the cell suspension at 250 × g for 5 min. Wash cell pellet with 500 μl of PBS and then resuspendedin QuickExtract solution. We typically use 50 μl for one well ina 24-well plate
High‐Throughput RT‐PCR for Small‐Molecule Screening Assays
One‐step qPCR kit (e.g., Roche Real‐time Ready cell lysis kit, cat. no. 05943523001) GO TO THE FULL PROTOCOL:
mNSC for the MTT Assay Basic Protocol 2: MTT Assay Support Protocol 4: Assay Validation for the MTT Assay with mNSC: Plate Uniformity and Signal Variability Basic Protocol 3: Preparation
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