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文献和实验E-Z 96 X-press Plasmid Vacuum Protocol
with swinging-bucket rotor capable of 3,000 x g 2. Vacuum Manifold (Cat# Vac-03) 3. Vortex 实验步骤 1. Pick up a single colony from a fresh streaked selective plate and Inoculate 2-3 mL LB medium containing the appropriate
Construction and Characterization of Adenovirus Vectors
) Tubes (polystyrene, conical, 15-mL) Tubes (polystyrene, round-bottom, 5-mL) Tubes (ultracentrifuge, Quick-Seal, 16- x 76-mm) (Beckman 344322) Tubes (ultracentrifuge, 14- x 89-mm) Ultracentrifuge Vortexer Water baths
Replication timing by density transfer
, leu2-3,112, ura3-52, his6 in an A364A background. I aim for about 32 x 106 cells per time point. That gives enough DNA for at least 3 blots, leaving enough leeway for errors. 2. When the cell density is 2 x 106 cells/ml (OD660 ~ 0.16
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