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文献和实验Concentration Module 1) To the sample from Step 5, above, add 250 μl Binding Buffer (L3) and 125 μl 96-100% ethanol. Mix well by vortexing. 2) Bind the sample from Step 1 containing Binding Buffer (L3) and ethanol to the spin column
Methyl DNA Immunoprecipitation
of Immunoprecipitated DNA 1. METHYL kit (cat. no. mc-green-003, Diagenode), which includes the Methyl DNA IP module (cat. no. mc-green-001) and the GenDNA and qPCR modules (cat. no. mc-green-002
nuclei, a cycling cell component that allows assessment of proper function of doublet discrimination module (DDM) or pulse processing. Please follow the instructions included in the kit for quality control and set-up of the machine. Acquire data
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