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文献和实验anaerobic organisms using FCM and 96-well plates for growth on biomass-relevant substrates. The method was tested using the cellulolytic thermophiles Clostridium thermocellum (T opt = 55 �C), Caldicellulosiruptor obsidiansis (T opt = 78 �C
potent reducing agents to reactivate the enzymes present; however, this approach does not allow for the measurement of the endogenous PTPase activity directly isolated from the in vivo cellular environment. Here we provide a method for using an anaerobic
and cultivation like for many of the numerous endosymbionts of protozoa. Examples are the endonuclear symbionts in ciliates that have been known for more than a century (1 ). Cells of the genus Holospora multiply in the macro- or micronucleus of Paramecium
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