1 L (1000 mL), Polypropylene B

Methods for DNA isolation

or cosmid DNA of interest into a 12 X 75 mm Falcon tube containing 2 ml of LB media supplemented with the appropriate antibiotic (typically ampicillin at 100 ug/ml) and incubate at 37deg C 8-10 hours with shaking at 250 rpm. Transfer the culture

Endothelial Cell Systems - Instructions For Use

-5022)1 x 100 ml bottle Trypsin/EDTA Solution (CC-5012) 1 x 100 ml bottle Trypsin Neutralizing Solution (CC-5002) 1 x 100 ml bottle NOTE: If you use a different Clonetics® medium, see Appendix A, Endothelial Cell Media Endothelial Cell Systems Media

Studies of the Ubiquitin Proteasome System

lyophilized powder in 25 µl of 40 mM DTT on ice, allow to solubilize 15 min; prepare fresh [γ‐32 P]ATP (GE Healthcare) 6000 Ci/mmol 20× thrombin stock solution (see recipe