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文献和实验or cosmid DNA of interest into a 12 X 75 mm Falcon tube containing 2 ml of LB media supplemented with the appropriate antibiotic (typically ampicillin at 100 ug/ml) and incubate at 37deg C 8-10 hours with shaking at 250 rpm. Transfer the culture
or cosmid DNA of interest into a 12 X 75 mm Falcon tube containing 2 ml of LB media supplemented with the appropriate antibiotic (typically ampicillin at 100 ug/ml) and incubate at 37deg C 8-10 hours with shaking at 250 rpm. Transfer the culture
Endothelial Cell Systems - Instructions For Use
-5022)1 x 100 ml bottle Trypsin/EDTA Solution (CC-5012) 1 x 100 ml bottle Trypsin Neutralizing Solution (CC-5002) 1 x 100 ml bottle NOTE: If you use a different Clonetics® medium, see Appendix A, Endothelial Cell Media Endothelial Cell Systems Media
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