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文献和实验Agrobacterium growth and transformation
. This is not very efficient (~200 colonies per microgram of DNA), but it is really easy.1. Pick a single colony of the Agrobacterium strain of choice and inoculate 3 ml of LB (or 2YT) in a 15 ml snap-cap tube (Falcon tube). Grow @ 30℃ overnight on a roller drum. Be sure
Worm PCR Pick one worm and place it in a 2.5 l drop of lysis buffer in the cap of a PCR tube. Close and centrifuge briefly to move to the bottom of the tube. Freeze the tubes at -70°C for 15 min. The idea is to do a freeze
Agrobacterium growth and transformation (农杆菌培养及转化)
snap-cap tube and incubate for ~2 hours on a roller drum @ 30°C. 7. Pour contets into a 1.5 ml microfuge tube and spin tubes 5 minutes at ~4K rpm to pellet cells. Remove supernatant and resuspend pellet in 100 ul of LB (or 2YT). 8. Plate
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