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文献和实验Plant DNA methylation analysis by bisulfite genomic sequencing
(12000 X g); Discard supernatant, vacuum dry for 10 minutes at room temperature; Dissolve cut DNA in 10µl bidist water (0.25 or 2.5 µg/µl, respectively), store at -20°C; Bisulfite treatment The bisulfite solution
Metallomics Using Inductively Coupled Plasma Mass Spectrometry
6 for anaerobic preparation) Vacuum manifold (connected to mechanical vacuum pump and argon tank) Stir
STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
the plants the day before infiltation). Draw vacuum for 15-20 min for WS and 30 min for Col-0 at a pressure close to 0.05 Bar (we are using an oil pump). 7. Before removing the plants from the vacuum jar place a plastic bag over the pot and beaker. Pull
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