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- Beckman Coulter贝克曼库尔特
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- 2025年07月11日
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文献和实验to eliminate platelets. 5) Centrifuge at 350 x g for 20 minutes at 20°C. Allow to decellerate without brakes. 6) Recover MNC from the plasma/Lymphoprep interface and transfer the cells to a 50 ml tube. 7) Wash MNC
Fastfilter Plasmid Midi Kit Spin Protocol
Column: 10. Transfer 4 ml of the clear lysate to the HiBind® DNA Midi column assembled in the 15 ml collection tube. The Midi column has a maximum capacity of 4.5 ml. Centrifuge at 3,000-5,000 x g for 3-5 min at room temperature to completely pass
Reprogramming Fibroblasts with the CytoTune-iPS Reprogramming Kit
°C 5. Sterile serological pipettes (5-mL, 10-mL) 6. Centrifuge 7. 15-mL centrifuge tubes 8. 60-mm and 100-mm tissue culture-treated dishes 9. 6-well tissue culture-treated plates 10. 25-gauge 1½-inch needle
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