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- Beckman Coulter贝克曼库尔特
- 美国
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- 2025年07月13日
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文献和实验! For preparative rotors, be sure the rotor cover is in place and properly screwed down, where appropriate. NEVER use a rotor without its lid, when one is supplied - the screw actually holds the rotor to the motor shaft. Check that the centrifuge chamber is clean
LABELING MUSCLE ACTIN WITH CARBOXYFLUORESCEIN SUCCINIMIDYL ESTER
. 6.While vortexing,add 166 µl (8.31 mg)CFSE stock solution to 1 ml of buffer 2. 7.Mix dye solution immediately with actin solution,by gentle pipeting or stirring.Incubate at room temperature for 1 hr. 8.Centrifuge in a 50Ti rotor at 40,000 rpm,4℃ for 1 hr
and properly screwed down, where appropriate. NEVER use a rotor without its lid, when one is supplied - the screw actually holds the rotor to the motor shaft. Check that the centrifuge chamber is clean, defrosted and that all membranes
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