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- Beckman Coulter贝克曼库尔特
- 美国
- B97759
- 2025年07月14日
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文献和实验【共享】经验:The RNeasy Mini Kit from Qiagen
Kit. Samples for RNA extraction initially need to be lysed and, if necessary, homogenized. An excellent accompanying handbook gives guidelines for RNA yields. For instance, yields for common cell culture lines (COS7, HeLa, etc) and different
DNA/RNA/Protein Purification from Cultured Cells Using SQ DNA/RNA/Protein Cell Kit (1-2 x 106 cells)
in this protocol. Collect suspended cells and place on ice until use. Determine the cell number by using a hemacytometer or other cell counter. 2. Preheat the water bath to 65°C 3. Water bath or heating block preset at 37°C 4. Pre-set
RNA Isolation From Animal tissue or cell culture
mL collection tube. The maximum capacity of the spin cartridge is 750 ul. A precipitate may form on addition of ethanol in step 5. Vortex and add the entire mixture to the column. With the spin column inside a 2ml collection tube (supplied with kit
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