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文献和实验Caution: EtBr is a powerful mutagen and is moderately toxic. Wear gloves when working. When EtBr conc. is >0.5 mg/ml: 1.Add sufficient water to reduce the concentration of EtBr to 2.Add 1 volume of 0.5 M KMnO4. Mix, add 1 volume of 2.5 N HCl
Silica Suspension: add 2 g of silica to 15 ml of H2O wash 3x by centifugation at 2000 x g for 2 min estimate vol of silica and resuspend in 2 vol H2O Silica Wash Solution: 50 mM NaCl,10 mM Tris 7.5,2.5 mM EDTA,50% Ethanol 6 M NaI note
column into a clean 1.5 mL micro centrifuge tube. 10) Add 15-20 ul Elution Buffer(10mM Tris, pH 8.5) or water to the center of the membrane, incubate at room temperature for 3-5 minute. 11) Centrifuge at maximal speed ($13,000 x g
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