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文献和实验Isolate DNA from NRBC Blood from Buccal Swab or dried blood spots collected with filter paper
,000 x g for 10 minutes. Carefully transfer the supernatant to a new 1.5 ml tube. 5. Add equal volume of absolute ethanol (room temperature, 96-100%) to the sample and mix by vortexing at maxi speed for 15 seconds. Collect any liquid drop from lid
Hybridization of High Density Arrayed BAC Nylon Filter Blots
to keep wet. Place filter blots down on film (Kodak BioMax MS, 8326886) into X-Omatic Regular (Kodak) cassette with regular screens. Note: you may add special MS screens for increased sensitivity. Incubate -80°C, overnight. Leave cassettes
Fluorochrome Absorption and Emission Spectra
, which are similar in structure to phycoerythrobilin, the chromophore in R-PE. It has a 650-nm wavelength absorption maximum (Fig. 2) and a 660-nm fluorescence emission maximum (Fig. 2). Using a 660 ?10 nm BP filter will give optimum detection for this fluorochrome
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