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文献和实验Plasmid Mini Purification Protocol
after addition of Buffer P3.Mix by inverting the tube 4-6 times. 4. Centrifuge at maximum speed in a microfuge for 10 min (or at 21,000 RPM in the F2402 rotor in the Beckman Avanti 30 centrifuge for 10 min).Remove supernatant promptly.Do step
Construction and Characterization of Adenovirus Vectors
before use. Equipment Agarose gel electrophoresis equipment Bottles (polypropylene, 500- and 1000-mL) Centrifuge (high-performance) (e.g., Avanti J-series, Beckman) Cryovials (4-mL) Dialysis cassettes (Slide-A-Lyzer, 10,000-kDa
DNA extraction from blister rust aeciospores and urediniospores using dry grindi
cold (-20 ℃) 70% ethanol, inverting tube several times. Centrifuge at top speed 5 min., pour off, centrifuge a few seconds, pull off remaining supernatant with a pipette tip. Dry pellet 30 min. in speed vac. 8) Resuspend pellet in 100 μl TE-8; heat
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