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文献和实验Fastfilter Plasmid Maxi Kit Spin Protocol
Column into a 50 ml collection tube, provided. Add 5 ml of Buffer GPS to the column and Lit it sit at room temperature for 3-10 min. Spin in a swinging bucket rotor at 3,000-5,000 x g for 5 minutes at room temperature. Discard the eluate and assemble
Fastfilter Plasmid Midi Kit Spin Protocol
Column: 10. Transfer 4 ml of the clear lysate to the HiBind® DNA Midi column assembled in the 15 ml collection tube. The Midi column has a maximum capacity of 4.5 ml. Centrifuge at 3,000-5,000 x g for 3-5 min at room temperature to completely pass
1. Weigh 500 mg of glass beads in a 2 ml centrifuge tube, add 0.2-0.5 g soil sample. Add 0.8 ml Buffer SLX Mlus. Vortex at maximum speed for 3-5 minute to lyse samples. For the best result, A Mixer Mill, such as Fastprep-24, Mixer Mill MM 300
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