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文献和实验Transplantation of Pancreatic Islets Into the Kidney Capsule of Diabetic Mice
into each microcentrifuge tube (~500 islets/tube/mouse). Allow islets to settle to the bottom of the microcentrifuge tubes. Draw one islet pellet into a P200 pipetman (set at 130ul) using a straight thin-wall pipet tip. Place a silicone tube adapter
General Considerations for Mouse Survival Surgery
Figure 1. Example of equipment and instrument set up for mouse surgery. Circulating water blanket (A ); clear surgical drape overlapping the sterile surgery field (B ); sterile field (C ); stereomicroscope (D ); goose neck lamp (E ); sterile saline
Native Chromatin Preparation and Illumina/Solexa Library Construction
et al. 2008 ), and SNP genotyping. Use of this protocol to analyze histone modification patterns is described in Barski et al. (2007) and Wang et al. (2008) . MATERIALS Reagents Adapter oligo mix (Illumina) (see Step 42)
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