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文献和实验Important : Extract the DNA within one week of receiving samples. Samples
Tail Preps: DNA Isolation From
is much better). 11. Pipet supernatant into a fresh tube. Avoid transferring the white precipitate. 12. Add 500µl isopropanol. 13. Mix by inversion until a white thready material is visible (DNA!). Shake until thread doesn't get any bigger
for a minimum of 50 PCR analyses, and the DNA is stable for at least one month in the refrigerator. A simple modification of this technique allows the isolation of DNA suitable for use in inverse PCR (Ochman et al, Genetics 120: 621-623). These methods
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