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文献和实验Preparation of Mitochondria from Rat Liver
, and 1 mM EDTA, pH 7.2 for homogenizing. We use a shearing type homogenizer called 'Tissuemizer,' with T25 stainless steel shaft, made by Tekmar, Inc. (Cincinnatti, Oh). A teflon-on-glass (Potter-Elvehjem) homogenizer can also be used, but it takes longer
called 'Tissuemizer,' with T25 stainless steel shaft, made by Tekmar, Inc. (Cincinnatti, Oh). A teflon-on-glass (Potter-Elvehjem) homogenizer can also be used, but it takes longer. The Polytron is fast but prolonged or overly vigorous homogenization can be damaging
Dissociated Retina Immunohistochemistry
slides, teflon coated) Procedure before starting see criteria/guidelines for dissociated cell scoring • Wash the tissue (freshly dissected or explant) in prewarmed PBS (6 ml in a 6 cm dish). • Transfer to an eppendorf tube
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