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- Beckman Coulter贝克曼库尔特
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文献和实验Growth, Maintenance and Transfection of Suspension Adapted 293-EBNA cells
prior to antibody purification VI. ASSOCIATED PROCEDURES A.Trypan blue viability test 1. Transfer 0.9 ml of 0.4% Trypan Blue + 0.1 ml of cell suspension in an Eppendorf Tube 2. Gently mix to avoid cell clusters by by pipetting up and down 3. Allow
Growth, Maintenance and Transfection of Suspension Adapted 293-EBNA cells
prior to antibody purification VI. ASSOCIATED PROCEDURES A.Trypan blue viability test 1. Transfer 0.9 ml of 0.4% Trypan Blue + 0.1 ml of cell suspension in an Eppendorf Tube 2. Gently mix to avoid cell clusters by by pipetting up and down 3. Allow
Fibroblast Cell Systems Instructions for Use
If your cell yield is low (less than 50%), determine the cause(s) and possible solution(s) using the table below. Then subculture one more flask applying the appropriate solution(s). If your cell viability is low (less than 50%), determine the possible
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