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文献和实验was discarded, and the cell pellet was reconstituted in 0.5 ml PBS (with Ca2+ ), placed within accessory sample vials (Cat no. 383721, Beckman-Coulter, Fullerton, CA, USA) and analyzed for cell viability with the trypan blue exclusion assay, by utilizing
Quantitation Kit (#F-2962, Molecular Probes, Eugene, OR) TE 12 channel multi-pipettors Computer equipped with Microsoft Excel software Ds-DNA Standards 50 µg/ml 100 µg/ml 250 µg/ml 500 µg/ml µl TE 90 80 50 0 µl ds-DNA (0.5 mg/ml) 10 20 50 100
【资源】全面归类网址:毕赤酵母表达各种来源蛋白(细菌,真菌,植物,人类等)
.,active[143Yellowjacket and paper wasp venom allergen A5 S, 100 mg/L, α-MF360Vertebrates (non-human)Bovine β-caseinI, 1 g/L[150]Bovine enterokinase catalytic domainS, 6.3 mg/L, α-MF[144]Bovine follicle stimulating hormone β-subunitS, 4 mg/ml, α-MF[145
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