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文献和实验ARABIDOPSIS THALIANA DNA ISOLATION PROCEDURE
ml of buffer A plus 0.5% Triton X-100 (Sigma) with a homogenizer (55 ml glass pestle unit). Centrifuge in two 30 ml Corex tubes at 8000 rpm for 10 min. in a Beckman JS-13 rotor. Repeat step 7, centrifuge at 6000 rpm for 10 min in a JS-13
David Harry Institute of Forest Genetics USDA Forest Service Pacific Southwest Research Station August 26, 1993 Background : There are many published methods for mini-preps of DNA from lambda phage cloning vectors. This one has worked
Chromatin Immunoprecipitation Protocol to Analyze Histone Modifications in Arabidopsis thaliana
using the Beckman JS 7.5 rotor (or equivalent) at 4000 rpm for 20 minutes at 4°C; Gently pour off supernatant and resuspend pellet in 1ml of Extraction Buffer 2 by pipetting up and down. Transfer solution to Eppendorf tube; Spin in cooled
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