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文献和实验剂(1% Ignite Hoechst-Roussel Agri-Vet Company, NJ) ( 5 ) 蛋白提取缓冲液:50 mmol/L 磷酸钠缓冲液(pH 7.0 ) ,10 mmol/L EDTA(乙二胺四乙酸), 0.1% (V/V) Triton X-100,0.1% (m/V ) Sarkosyl,10 mmol/L 疏基乙醇,10 mmol/L PMSF (phenylmethylsulfonyl fluoride)。 ( 6 ) 碱性
and centrifuged (14,000 x g) for 20 min. The pellet was air dried" and resuspended in 100 l of TE. DNA from both procedures was quantified using a minifluorometer Hoefer TKO 100). The RAPD protocol of Williams et al. (11) was modified "as follows
Genotyping in the Cloud with Crossbow
computer, and on the Amazon Elastic MapReduce cloud computing service. Curr. Protoc. Bioinform. 39:15.3.1?15.3.15. © 2012 by John Wiley & Sons, Inc. Keywords: short reads; read alignment; SNP calling; cloud computing; Hadoop; software package
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