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文献和实验on the biological and mechanical requirements of the target tissue/organ. Input parameters include the individual polyhedral units and overall scaffold block as well as the scaffold strut diameter. Taking advantage of its repeatability and reproducibility
product is detected in the PCR process as a significant increase in fluorescence, and the lower the Ct value. Ct values are very reproducible in replicates because the threshold is picked to be in the exponential phase of the PCR, where there is a linear
Introduction to the EMSA (Gel Shift) Technique
EMSA Reaction Parameters Target DNA Typically, linear DNA fragments containing the binding sequence(s) of interest are used in EMSAs. If the target DNA is short (20-50 bp) and well defined, complementary oligonucleotides bearing the specific
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