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- 2025年07月09日
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文献和实验for a decrease of fluorescence lifetime. Total enhancement is the product of these two effects. The recent reviews (14 , 15 ) contain more details on MEF. 2. Model Immunoassays To demonstrate a metal-enhanced fluorescence immunoassay on the glass
Transgenic Mouse Core Facility
precipitate Add 1/10th volume 3M NaOAc, pH=6.0 to aqueous layer. Add 2X volume EtOH. -20oC for at least 15 min. Microfuge at 4oC X 15 min. Remove EtOH. Air Dry. Resuspend each pellet in 50ul 10mM Tris, pH=7.5, 0.1mM EDTA. Combine into 1 tube
Study of Nucleolar Localization of Adenovirus Core Proteins
This chapter describes the techniques used to study nucleolar-localized proteins. The chapter starts with cloning of viral proteins for expression in mammalian cells as fusion proteins to well-characterized tags such as enhanced green
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