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- Beckman Coulter贝克曼库尔特
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- 2025年07月08日
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文献和实验per square by 104 to get the number of cells per ml of the original cell suspension. Determine the grand total number of cells available for subculturing , multiply the number of cells per ml by the total number of mls
per square by 104 to get the number of cells per ml of the original cell suspension. Determine the grand total number of cells available for subculturing , multiply the number of cells per ml by the total number of mls
Purification of Plasmid from 50 ml-culture
4. Resuspend the pellet in 5 ml of Solution I. 5. Add 10 ml of Solution II. Mix well by inverting the bottle more than 10 times. The solution should be clear after mixing Solution II. 6. Add 7.5 ml of Solution III. Mix well as above. 7. Spin
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