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文献和实验Genetic Transformation of Wheat via Particle Bombardment
Since its first invention in the late 1980s the particle gun has evolved from a basic gunpowder driven machine firing tungsten particles to one more refined which uses helium gas as the propellant to launch alternative heavy metal particles
Microinjection of dsRNA into Mouse Oocytes and Early Embryos
: A Guide to Gene Silencing (ed. Hannon). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2003. INTRODUCTION This protocol describes how to introduce a double-stranded RNA (dsRNA) of choice into mouse oocytes or fertilized
Amicon Stirred Ultrafiltration Cells
Description For protein concentration, gas pressure is applied directly to ultrafiltration cell. Solutes above the membrane's molecular weight (MW) cut-off are retained in cell, while water and solutes below the cut-off pass
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