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- Beckman Coulter贝克曼库尔特
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- 2025年07月09日
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文献和实验Fluorochrome Absorption and Emission Spectra
daltons and an absorption maximum at 495 nm (Fig. 2). Its excitation by 488-nm light leads to a fluorescence emission maximum around 520 nm (Fig. 2). Using a 530 ?15 nm bandpass (BP) filter will give optimum detection for this fluorochrome
Fluorochrome Absorption and Emission
daltons and an absorption maximum at 495 nm (Fig. 2). Its excitation by 488-nm light leads to a fluorescence emission maximum around 520 nm (Fig. 2). Using a 530 ?15 nm bandpass (BP) filter will give optimum detection for this fluorochrome
Fluorescein labeling of proteins
as preservative. Determine concentration and F/P ratio by measuring absorbance at 280 nm and at 495 nm.For antibodies,use the following formulae to get approximate values: Protein concentration (mg/ml)= (OD280 - 0.35 x OD495)/ 1.4 F/P ratio = (3.3 x OD495)/ (OD
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