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文献和实验E.Z.N.A. Endo-Free BACs, PACs, P1s Protocol
3 for detail instructions. 10. Apply the sample to the HiBind® DNA column assembled in a 2 ml collection tube (provided). 11. Centrifuge at > 8,000 x g for 30 second at room temperature. Remove the HiBind® DNA column, discard the flow-through and re-use
Construction and Characterization of Adenovirus Vectors
) Tubes (polystyrene, conical, 15-mL) Tubes (polystyrene, round-bottom, 5-mL) Tubes (ultracentrifuge, Quick-Seal, 16- x 76-mm) (Beckman 344322) Tubes (ultracentrifuge, 14- x 89-mm) Ultracentrifuge Vortexer Water baths
Cell-free System for the examination of apoptotic activity in cytoplasmic extracts
. from Jurkat cells) were added. The reactions were incubated at 37°C for 4 h. Here is an example for a reaction mix for this kind of experiment: Pipet x µl of DB into a microfuge tube.
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