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文献和实验Constructing a Low-budget Laser Axotomy System to Study Axon Regeneration in C. elegans
AJS100-0.5K 4 Thread adaptor, ?-20 male, 8-32 female Newport SS-1-B 2
Native Chromatin Preparation and Illumina/Solexa Library Construction
) Phusion HF Master Mix (2X, Illumina) (see Step 45) Polymerase chain reaction (PCR) primers PE 1.0 and PE 2.0 (Illumina) (see Step 45) Primer mix for real-time PCR (see Step 38) Several companies offer primer synthesis service.
adapt 3-4 week old plants (just beginning to bolt) for 2-3 d, then harvest tissue (1-4 rosette leaves) into 1.5 mL microfuge tubes w/ liquid N2. Grind tissue on liquid N2 with pellet pestle (Kontes Scientific Glassware/Instruments; cat #749520-9001
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