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文献和实验Native Chromatin Preparation and Illumina/Solexa Library Construction
the residual platelets. Remove supernatant, resuspend cells in 1X PBS, and repeat the wash once to remove remaining platelets. 4. Resuspend in 20-30 mL of 1X PBS and then count the cells. Aliquot 0.5 x 106 cells for fluorescence-activated
Differential cDNA Screening Procedures
in ethanol ice bath. (b) FIRST-STRAND SYNTHESIS (After Brown and Kafatos, 1988: J.Mol,Biol, 2O3:425-437) 1. To the annealed oligo and poly(A)+ RNA add the following on ice: 1.25X RT Buffer (-CTP) 80ul 5-methyl dCTP 10
with the MagNA Pure LC total nucleic acid isolation kit. J. Clin. Microbiol. 43, 4616–22. 8. The 10 categories of HCRW WHO Healthcare waste management (HCWM). (Accessed January 25, 2010, at http://www. healthcarewaste.org/en/128_hcw_categ. html).
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