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文献和实验Autoradiography using Emulsion-coated Coverslips
.4). In the dark, open the box of coverslips and, holding the coverslips only at the undipped end, carefully place one coverslip on each slide. The emulsion coated end of the coverslip should extend about 2 mm over the end of the slide
Plant DNA methylation analysis by bisulfite genomic sequencing
reaction tube; Add 0.5ml chloroform, vortex; Centrifuge as in step 7; Transfer the upper aqueous phase to clean 1.5ml reaction tube; Add 35µl 3M NaOAc/HOAc pH 5.0; 850µl abs. ethanol; Incubate >1 hour at -20°C
Constructing a Low-budget Laser Axotomy System to Study Axon Regeneration in C. elegans
to control focus 1) This system uses dual Galilean beam expanders to expand the laser beam to fill the back aperture of the objective (10 mm). Mount the 4 lenses on to carriers and attach them to the rail (L1f1 L2f2 and L3f1' L4f2'). Adjust
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