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- Beckman Coulter贝克曼库尔特
- 美国
- C14294
- 2025年07月09日
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文献和实验Purification of Plasmid from 50 ml-culture
of cold 70% ethanol. Discard the fluid. 14. Add to the pellet 400 ul of TE containing DNase-free RNase A (20 ug/ml). Incubate the tube for 30 min at 37 C. 15. After 30 min, carefully check the content of the tube. If nucleic acid pellet
E-Z 96 X-press Plasmid Vacuum Protocol
selective antibiotic. Incubate 14-16 hours at 37℃ 600 with vigorous shaking until an OD of 2.0-4.0 is achieved. 2. Pellet 1-2 mL bacterial culture in a 2.0 ml 96-well deep-well plate by centrifugation at 3000 x g for 15 min at room temperature.
E.Z.N.A. Endo-Free BACs, PACs, P1s Protocol
. Place the HiBind® DNA column back into the collection tube and centrifuge at maximum speed for 2 minutes to dry the column. 14. Place the HiBind® DNA column into a clean 1.5 ml centrifuge tube, apply 40 ul of Elution Buffer (10mM Tris-HCl, pH
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