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文献和实验David Harry Institute of Forest Genetics USDA Forest Service Pacific Southwest Research Station August 26, 1993 Background : There are many published methods for mini-preps of DNA from lambda phage cloning vectors. This one has worked
Agrobacterium growth and transformation
from the Bio-Rad machine is also pre-chilled on ice. 4. Take the ice bucket with the cuvettes and cuvette holder to the gene pulser. For cuvettes with a 2mm gap size, adjust the Gene Pulser unit "Set Volts"setting to 2.5 kV and the "CAP" setting to 25 uFD
Agrobacterium growth and transformation (农杆菌培养及转化)
plasmid that possesses the vir genes needed for T-DNA transfer, but has no functional T-DNA region of its own. Grow at 28-30°C. Store as glycerol stock (800 ml of fresh overnight culture + 200 ul sterile 80% glycerol) at ¬80°C. Transformation
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