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文献和实验Transplantation of Pancreatic Islets Into the Kidney Capsule of Diabetic Mice
. While maintaining the kink, disconnect the silicone adapter tubing from the pipetman and secure the kink with the silicone adapter. Place the islets, in the PE50 tubing, kinked silicone adaptor tubing side down, into a 15 mL conical with a cut 5 mL pipet
off with M9 and put them in a 15ml polypropylene tube. 4) Spin the worms in a clinical centrifuge at 2500 RPM for about 30 seconds (or just let gravity-settle for 15 minutes on ice) and transfer the worms in about 1ml to an eppendorf tube.
【交流】染色体免疫沉淀技术详细步骤-young lab !!!
LB3. 4. Transfer cells to a homemade “sonication tube” (cut a polypropylene, 15ml conical tube into two pieces at the 7 ml mark) 5. Sonicate suspension with a microtip attached to Misonix 3000 sonicator. Samples should be kept in an ice water bath
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