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文献和实验Purification of Plasmid from 50 ml-culture
. Transfer the supernatant containing plasmid DNA to a new microfuge tube. Add 600 ul of isopropanol. Mix well, and then spin the solution at 14,000 rpm for 5 min at 4 C. 13. Discard the supernatant. Rince the pellet and the wall of the tube with 500 ul
ES CELL DNA EXTRACTION: TUBE ME
off supernatant as in step 16. 19. Store the open tube on the bench at TRm until the last traces of fluid have evaporated. (i.e.: Air dry). 20. Dissolve the DNA in 30µl (or 50 µl, and use half of sample) of 10mM Tris pH 8.5 (or TE). (pipet
ES CELL DNA EXTRACTION: TUBE ME
Protocol for extracting DNA from ES Cells, starting from the 96-well plate but processing in an eppendorf tube to recover more of the DNA . NOTE- THIS TAKES A LOT OF TIME if you do the whole plate this way! Lysis buffer: ( 100 ml Recipe ) 10 mM
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