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文献和实验Mag-Bind® mRNA Protocol (Standard Protocol)
capable of 12,000 x g and 2-8°C 实验步骤 Total RNA Isolation: 1. Homogenization and lysis of samples: follow either method below. 1) Tissue Samples Homogenize tissue samples in 1.5 mL of RNA-Solv ® Reagent per 100
E.Z.N.A.® Dried Blood DNA Spin Protocol
speed for 20 seconds. 7. Transfer the lysate from step 5 into the column, and centrifuge at 10,000 x g for 1 min to bind the DNA. Discard the flow-through liquid and the collection tube. 8. Place the HiBind® DNA Mini Column into a NEW 2 ml
E-Z 96 X-press Plasmid Vacuum Protocol
collection plate as a support to give the collection plate a proper position. 15. Add 100-150 μl Elution Buffer (10mM Tris-HCl, pH 8.5) or sterile water to each well of the E-Z 96® X-press Plate, let stand for 2 minutes. Apply maximum vacuum for 5-10
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