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文献和实验Prehybridization and probe labeling
to each column and centrifuge for 4 minutes at 1,500 RPM. Probe purification Add 185 µL of TE to each probe. Cut the cap off the tube and withdraw all of the probe solution from the tube with a pipet. Place an empty tube
Screening Colonies by Hybridization with Radiolabeled Probe
solution per bottle (42°C with formamide, 68°C without formamide). Prehybridize for 1 - 2 hours. Boil probe for 5 minutes. Make sure the cap of the tube is secured to avoid radioactive spill when tube pops open due to heat expansion! Spin
UPDATED DIRECTIONS FOR NEW BRUNSWICK MOBILE PILOT PLANT FERMENTOR
, turn selector switch to D.O. and mode switch to zero. Set at value at zero. Remove grounding cap from D.O. probe and connect D.O. line. Set mode switch to span. when D.O. level has stabilized, set at 100%. Rotate mode switch to set point and enter value
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