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文献和实验Assessment of Acute Thermal Nociception in Laboratory Animals
Models of acute nociception using a thermal stimulus are widely employed as screening methods for nociceptive properties of new drug compounds. In this chapter, detailed descriptions for conducting of two of the most commonly used models
Replication timing by density transfer
Replication timing by density transfer M. K. Raghuraman 1. Grow cells at least 7 generations in 0.1% (w/v) [13
(with or without stirring). Cells are collected by centrifugation and washed twice with sterile water, and then cells are plated at about 5X10 6 cells per plate on cycloheximide medium ( 3.0 µg/ml cycloheximide) selective for the YAC transfer. YACductants
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