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- Beckman Coulter贝克曼库尔特
- 美国
- 2084149-01
- 2025年07月15日
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文献和实验and redissolve in 20 µl H 2ODEPC measure RNA concentration and check integrity on a denaturing gel II. cDNA synthesis for each RNA set up four reactions (one tube for each degenerate anchored oligo(dT) primer set - T 12MA,T12 MC
. Do not disturb the resin. Spin column for 2 minutes @ 735xg. Discard column in radioactive waste. Take out 1 μl of labeled probe in 0.5 ml eppendorf tube, put tube in scintillation vial and count the cpm. Count:_______________ UV
Two-hybrid analysis of genetic
and prey strains (see Protocol 1) One set of the following 150 x 15 mm plates for each test of interactions between an activation domain-tagged protein (in a prey strain)and 96 baits (bait strains): -u-h- Glu; -w Glu; YPD; -u-h-w Glu X-Gal; -u-h-w Gal/Raf
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