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文献和实验Microprinting of Liver Micro-organ for Drug Metabolism Study
configuration and composition of cells and bioactive matrix components can establish the well-defined biomimetic microenvironments that promote cell–cell and cell–matrix interactions. With the advent and refinements in microfabricated systems which can present
ORNL MICROARRAY HYBRIDIZATION PROTOCOLS
Direct labeling of total RNA with Cy3 and Cy5: A. MATERIALS RNeasy® Mini Kit (Qiagen; Cat # 74106) SuperScript II RT (200U/µL) (Life Technologies; Cat # 18064-014) QIAquick PCR Purification Kit (Qiagen; Cat # 28106)
,被pi染色后能被共聚焦显微镜观察。本片显示的绿色荧光(外源性凝集素)勾勒出凋亡细胞表面的小泡,红色荧光(pi)被包被在其中。 Apoptosis induced in Jurkat cells with 10 µM camptothecin. The cells were then treated with the reagents in the Vybrant Apoptosis Assay Kit #4 (V13243). Apoptotic cell nuclei
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