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- Beckman Coulter贝克曼库尔特
- 美国
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- 2025年07月14日
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文献和实验Rapid Retrieval of DNA Target Sequences by Primer Extension Capture
in a few samples, hybridization capture approaches such as those using microarrays currently involve bulky handling steps, long incubation times, and high per-sample costs. In contrast, the primer extension capture (PEC) method allows direct selection of small
to under 30°C. Add 18.5µl of DEPC treated water. Add 20µl of 5* Superscript II buffer. Add 10µl 0.1 M DTT. Add 20µl dNTP-mix for primer extension. Add 1µl of RNasin (RNase-inhibitor, Promega). Add 0.5µl Superscript II
Convergent Extension Analysis in Mouse Whole Embryo Culture
4 somite stage, leading to an almost entirely open neural tube (termed craniorachischisis). Studies in whole embryo culture have identified a defect in the morphogenetic process of convergent extension during gastrulation, preceding the onset of neural tube
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