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- Beckman Coulter贝克曼库尔特
- 美国
- 2087864-01
- 2025年07月12日
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文献和实验min. Transfer supernatant to 30ml tube, add 12 ml of room temperature ethanol. Pellet DNA by centrifugation at 15,000g, 15 min. Resuspend in 3.0ml TE, add 3.87g CsCl, make up to 4.9ml. After CsCl is dissolved (by swirling), add
Chromatin Immunoprecipitation from Mammalian Cell Extracts
them through a 21 guage needle.Add 100ml 10mg/ml PMSF and incubate on ice for another 30min.Transfer lysate to 2ml microcentrifuge tubes (1ml lysate/tube). 5.Shear chromatin Using a Branson 350 Sonifier with a microtip at a power setting of 7 and a 60% duty cycle
Chromatin Immunoprecipitation Protocol to Analyze Histone Modifications in Arabidopsis thaliana
Add 37ml 1% formaldehyde solution. Gently submerge seedlings at the bottom of the tube by stuffing the tube with nylon mesh. Screw on cap and poke cap with needle holes. Put in exsiccator and draw vacuum for 10 minutes; Release vacuum
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