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文献和实验. 4). 10) Place a sterile syringe filter into the air vent (Fig. 4). 11) Move the flow circuit into a humidified incubator at 37 °C, 5% CO2 . 12) Clamp the hard tubing into the roller pump head indicated for use with this type
Solutions for Tissue/Cell Digestion
Trypsin (13 ug/ml) in Saline G Materials Worthington 3× crystallized trypsin (LS03707), 1.3 mg. Sterile Saline G (pH 7.0), 100 ml. Procedure (all steps on ice) 1. Dissolve 1.3 mg trypsin in 100 ml Saline G. 2. Sterile filter
Solutions for Tissue/Cell Digestion
Solutions for Tissue/Cell Digestion Trypsin (13 ug/ml) in Saline G Materials Worthington 3x crystallized trypsin (LS03707), 1.3 mg. Sterile Saline G (pH 7.0), 100 ml. Procedure (all steps on ice) 1. Dissolve
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