1L (1000mL) , Polycarbonate Bo

常用缓冲液配制

in screw-cap vial, store at -20 C. 10 X REact buffer 3 : for, e.g. Eco RI (5.0 ml) 1 X Conc Stock 500 mM 1M Tris (pH 8.0) 2.5 ml 100 mM 1M MgCl2 0.5 ml 1 M 5 M NaCl 1.0 ml 25 mM Spermidine(3HCl) 0.032 g H2O 1.0 ml Mix, aliquote 2 ml

C. elegans RNA prep

clear, add 175 ml of TE + 0.1% SDS to each tube, and suspend the RNA by pipetting up and down. This should take about 1 minute. Transfer the RNA to a fresh RNase free screw cap eppendorf tube. Wash out the bottom of the ultracentrifuge tube

秀丽线虫RNA提取--C. elegans RNA prep

. 5. Pour the mixture into a 28 ml polycarbonate Oak Ridge tube. Spin in the 70Ti ultracentrifuge rotor for 20 minutes at 30K RPM at 20° to pellet debris (and any metallic stuff from the polytron). Pour off the supernatant into a clean 50 ml