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- Beckman Coulter贝克曼库尔特
- 美国
- 2088664-12
- 2025年07月11日
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文献和实验Transformation of E. coli by Electroporation
absolute ethanol. Ice 15 min. 3. Centrifuge at >12,000 x g for 15 min at 4° C. Carefully decant the supernatant. 4. Wash the pellet with 1 ml of 70% ethanol. Centrifuge at >12,000 x g for 15min at room temperature. Remove the supernate. 5. Air
Transformation of E. coli by Electroporation电穿孔转化E.coli
, discard supernatant and resuspend cell pellets in 2 x 125 mL 10% glycerol. FROM HERE ON, ALWAYS KEEP CELLS COLD!!! 6.Centrifuge as before; discard supernatant and resuspend as before; recentrifuge and discard supernatant 7.In cold room or on ice
4.Centrifuge at 2600g for 15 min in a 4 ℃ superspeed centrifuge 5.In cold room or on ice, discard supernatant and resuspend cell pellets in 2 x 125 mL 10% glycerol. FROM HERE ON, ALWAYS KEEP CELLS COLD!!! 6.Centrifuge
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