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文献和实验ES CELL DNA EXTRACTION: TUBE ME
in an eppendorf tube to recover more of the DNA . NOTE- THIS TAKES A LOT OF TIME if you do the whole plate this way! Lysis buffer: ( 100 ml Recipe ) 10 mM Tris-HCl pH7.5 - 0.5 ml of 2M 10 mM EDTA - 2 ml of 0.5M 10 mM NaCl - 0.2 ml
ES CELL DNA EXTRACTION: TUBE ME
Protocol for extracting DNA from ES Cells, starting from the 96-well plate but processing in an eppendorf tube to recover more of the DNA . NOTE- THIS TAKES A LOT OF TIME if you do the whole plate this way! Lysis buffer: ( 100 ml Recipe ) 10 mM
ES CELL DNA EXTRACTION: TUBE METHOD
of each well into a separate 1.5ml eppendorf tube (µ-cent. tube). 4. Continue lysing the cells for 2 more hours. (60°ree;C). (Float tubes in waterbath- don't need tupperware). 5. Add an equal volume (250 µl) of phenol:chloroform (phenol: chloroform: isoamylalcohol, 25
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