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文献和实验Purification of Plasmid from 50 ml-culture
1. Shake E. coli harboring plasmid at 37 C overnight in 50 ml of TB containing appropriate antibiotics. (when using ampicillin, addition of the antibiotics to 100-200 ug/ml rather than usual 50 ug/ml may improve the yield of plasmids.)
:GLASS BEADS 下一篇:Salmon Sperm DNA (10mg/ml)
SQ Blood DNA Maxi Protocol for 4-10 ml whole blood
, and then repeat Step 7. 8. Transfer the supernatant to a new nuclease-free 15 ml centrifuge tube containing one volume of 100% isopropanol. Do not transfer the protein pellet. 9. Gently mix the solution by inverting the tube 40-50 times. 10
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