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文献和实验12 mL of Plasmodium falciparum blood culture at 4% hematocrit Equipment Refrigerated centrifuge capable of holding 15 mL conical tubes Microcentrifuge Procedure Centrifuge parasite culture at 1400 rpm (~484xg
Cell Lysis/Western/IPSteven Finkbeiner,Departments of Neurology and Physiology,UCSFcompiled by 6/95 by AJS from AB)Fresh 1X LysisFresh 1X LysisFinal5ml10ml2X Lysis 2.5 ml5mlNaCl0.25M0.25ml of 5M0.5 ml of 5MNa3VO40.1mM25l (μl) of 200 mM5l (μl) of 200
9M Urea,2.5mM EDTA,2.5mM EGTA,1% DTE,4% CHAPS make 10ml and aliquot 10x1ml,freeze at -70℃ Lysate preparation wash the cells 2x with PBS wash the cells 1x with 10mM Tris,250mM Sucrose lyse the cells with 100 – 350μl of urea lysis buffer
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